Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 10. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 11. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 12. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Clinical Epigenetics

Article Title: Abnormal DNA methylation analysis of leucine-rich glioma-inactivated 1 antibody encephalitis reveals novel methylation-driven genes related to prognostic and clinical features

doi: 10.1186/s13148-023-01550-5

Figure Lengend Snippet: A A cluster heatmap showing methylation changes in promoter of 13 microRNAs between LGI1 encephalitis cohort and normal donors. B Morphological characteristics of apoptotic body, microvesicles, and exosome isolated from serum of one LGI1 encephalitis patient by TEM. C Particle size of microvesicles and exosome using NTA. D Protein markers of apoptotic body (C3B, C1QC), microvesicles (ARF6), exosome (CD63, TSG101) and serum supernatant by western blot. E Volcano plot of differentially expressive exosome-microRNAs between LGI1 encephalitis and healthy control. F A scatter plot assessing the expression variation of exosome microRNAs between LGI1 encephalitis patients and healthy donors. G Venn diagram showing the overlap of 71 differential exosome microRNAs and 13 methylated-driven microRNAs. H The methylation changes in promoter of hsa-miR-2467-5p between LGI1 encephalitis cases and healthy donors. I PCR analysis of miR-2467-5p expression in PBMCs between LGI1 encephalitis patients and healthy donors. J PCR analysis of miR-2467-5p expression in apoptotic bodies isolated from LGI1 encephalitis patients and healthy donors. K PCR analysis of miR-2467-5p in microvesicles isolated from LGI1 encephalitis patients and healthy donors. L PCR analysis of miR-2467-5p in exosomes isolated from LGI1 encephalitis patients and healthy donors. M Schematic representation of the complementary binding sites of CSF3 and PDCD1 with miR-2467-5p. Relative luciferase activity of wild-type and 3`UTR mutant constructs of CSF3 N and PDCD1 O cotransfected with miR-2467-5p mimics and miRNA-NC. PCR analysis of PDCD1 P , CSF3 Q and CCL15 R expression in PBMCs after transfection of miR-2467-5p mimics, inhibitor or scramble control into PBMCs. ELISA analysis of PDCD1 S , CSF3 T, and CCL15 U expression in cell supernatants after transfection of miR-2467-5p mimics, inhibitor or scramble control into PBMCs. Scatterplots showing the association of miR-2467-5p in PBMCs with the expression of PDCD1 V , CSF3 W , and CCL15 X in serum in LGI1 encephalitis patients ( n = 6) and healthy donors ( n = 4). LGI1-E LGI1 encephalitis; ND normal donor; AE autoimmune encephalitis; MV microvesicles; ABs apoptotic bodies; Sup supernatant; NC negative control. Data are means ± SD of three experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: ELISA kits were used to measure CSF3, PDCD1, and CCL15 according to the manufacturer's instructions (Boster, Wuhan, China).

Techniques: Methylation, Isolation, Western Blot, Control, Expressing, Binding Assay, Luciferase, Activity Assay, Mutagenesis, Construct, Transfection, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Clinical Epigenetics

Article Title: Abnormal DNA methylation analysis of leucine-rich glioma-inactivated 1 antibody encephalitis reveals novel methylation-driven genes related to prognostic and clinical features

doi: 10.1186/s13148-023-01550-5

Figure Lengend Snippet: PDCD1 and CSF3 expression level in thymoma. A and B PDCD1 and CSF3 expression were compared between thymoma and normal tissues. C and D PDCD1 and CSF3 expression in thymoma were indicated in Masaoka stage I–IV. E and F PDCD1 and CSF3 expression in histological type A–C of thymoma. G and H PDCD1 and CSF3 expression in different tumor sites. All expression value (TPM value) were acquired using the TCGA dataset on the Xiantao platform. ns no significant. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: ELISA kits were used to measure CSF3, PDCD1, and CCL15 according to the manufacturer's instructions (Boster, Wuhan, China).

Techniques: Expressing

Journal: Clinical Epigenetics

Article Title: Abnormal DNA methylation analysis of leucine-rich glioma-inactivated 1 antibody encephalitis reveals novel methylation-driven genes related to prognostic and clinical features

doi: 10.1186/s13148-023-01550-5

Figure Lengend Snippet: The association of PDCD1 and CSF3 expression with thymoma immune infiltration. A The expression of IFNγ, ICAM1, TNF and CCL2 were compared between thymoma and normal tissues using the TCGA dataset on the Xiantao platform. B Spearman correlation were shown between PDCD1 and CSF3 expression in thymoma. C and D The correlations of PDCD1 and CSF3 with all 24 immune cell types. iDC immature DC; Tem T effector memory; TFH T follicular helper; Tgd T gamma delta; pDC plasmacytoid DC; aDC activated DC; Tcm T central memory. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: ELISA kits were used to measure CSF3, PDCD1, and CCL15 according to the manufacturer's instructions (Boster, Wuhan, China).

Techniques: Expressing

Journal: PLoS Genetics

Article Title: CSF1R-dependent macrophages control postnatal somatic growth and organ maturation

doi: 10.1371/journal.pgen.1009605

Figure Lengend Snippet: (A) Representative images showing staining for IBA1 expression (brown) in naive 7-week-old WT and Csf1rko rat tibiae. Original magnification: 40X. Scale bar: 50μm. (B) Flow cytometry gating strategy to distinguish (i) CD172A+ myeloid cells, (ii) HIS48 + /SSC Hi granulocytes among myeloid cells, (iii) HIS48 and CD43-expressing monocyte subsets among non-granulocyte myeloid cells and (iv) CD45R + B cells among CD172A - lymphocytes. (C-E) Quantification of peripheral blood (C) granulocytes, (D) monocyte subsets and (E) B cells in WT and Csf1rko rats at 3 and 9 wks of age. (F) Bone marrow flow cytometry gating strategy to distinguish (i) HIS48 + /SSC Hi granulocytes, (ii) CD172 + and CD172 Low /CD4 Int monocytes/macrophages among non-granulocytes, (iii) monocyte/macrophage subsets and (iv) CD45R + B cells among CD172A - /CD4 - cells. (G-I) Quantification of BM (G) granulocytes, (H) monocyte/macrophage subsets (colours correspond to populations indicated by arrows and boxes in the FACS profiles) and (I) B cells. All genotype comparisons were analysed by Mann Whitney test.

Article Snippet: Serum CSF1 and CSF3 were analysed using Rat CSF1 SimpleStep ELISA kit (Abcam, Australia, Cat# ab253214) and Rat Granulocyte Colony Stimulating Factor (G-CSF/CSF3) ELISA Kit (MyBioSource, San Diego, CA, USA, Cat# MBS265555) respectively according to manufacturer’s instructions.

Techniques: Staining, Expressing, Flow Cytometry, MANN-WHITNEY

Journal: PLoS Genetics

Article Title: CSF1R-dependent macrophages control postnatal somatic growth and organ maturation

doi: 10.1371/journal.pgen.1009605

Figure Lengend Snippet: (A) Flow cytometry gating strategy to identify peritoneal granulocytes and macrophages. (B, C) Flow cytometric analysis of (B) peritoneal granulocytes and (C) macrophages in Csf1rko rats 9 wks post BMT at 3 wks of age compared to age-matched WT controls. (D-F) Flow cytometric analysis of peripheral blood (D) monocyte subsets, (E) granulocytes and (F) B cells in Csf1rko rats 9 wks post BMT at 3 wks of age compared to age-matched WT controls. (G) Representative images showing dual staining for IBA1 expression (brown) and TRAP activity (magenta) in the tibiae of Csf1rko rats at 6-weeks post-BMT (right) and aged matched WT control (9 weeks) (left). Original magnification: 40X. Scale bar: 50μm. (H-J) Flow cytometric analysis of bone marrow (BM) (G) monocyte/macrophage subsets, (H) granulocytes and (I) B cells in Csf1rko rats 9 wks post BMT at 3 wks of age compared to age-matched WT controls. (K) BM from WT and Csf1rko rats shown in panels G-H was cultured for 7 days in 100 ng/ml CSF1 to generate bone marrow-derived macrophages (BMM). Images shown are representative of cultures from 5 separate WT and BMT recipient Csf1rko animals (scale bar 50 μM). All genotype comparisons were analysed by Mann Whitney test.

Article Snippet: Serum CSF1 and CSF3 were analysed using Rat CSF1 SimpleStep ELISA kit (Abcam, Australia, Cat# ab253214) and Rat Granulocyte Colony Stimulating Factor (G-CSF/CSF3) ELISA Kit (MyBioSource, San Diego, CA, USA, Cat# MBS265555) respectively according to manufacturer’s instructions.

Techniques: Flow Cytometry, Staining, Expressing, Activity Assay, Control, Cell Culture, Derivative Assay, MANN-WHITNEY